Development of cultivation technique for pure Plasmodium falciparum gametocytes.
Southeast Asian J Trop Med Public Health
;
1995 Dec; 26(4): 606-10
Article
Dans Anglais
| IMSEAR
| ID: sea-33507
ABSTRACT
Pure gametocyte culture of Plasmodium falciparum, isolate KT3, from Kanchanaburi Province, Thailand, was successfully established by 5% sorbitol treatment on days 9, 10 and 11 following initiation of culture. Using medium supplemented with 15% human plasma and activated erythrocytes, daily medium change was not required during the cultivation. There was 99% reduction in the numbers of asexual parasites in the culture but the numbers of gametocytes were not affected. Furthermore, the gametocytes could undergo their usual morphological development with retention of function as demonstrated by the appearance of exflagellating microgametocytes, macrogametocytes and of oocyst formation in midgut of infected mosquito.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Plasma sanguin
/
Plasmodium falciparum
/
Sorbitol
/
Facteurs temps
/
Femelle
/
Humains
/
Mâle
/
Paludisme à Plasmodium falciparum
/
Milieux de culture sans sérum
/
Érythrocytes
langue:
Anglais
Texte intégral:
Southeast Asian J Trop Med Public Health
Année:
1995
Type:
Article
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