Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
Southeast Asian J Trop Med Public Health
;
1989 Dec; 20(4): 519-22
Article
Dans Anglais
| IMSEAR
| ID: sea-34669
ABSTRACT
A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Radio-isotopes du phosphore
/
Plasmodium falciparum
/
Humains
/
Sondes d'ADN
/
Méthodes épidémiologiques
/
Animaux
/
Paludisme
/
Hybridation d'acides nucléiques
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
Southeast Asian J Trop Med Public Health
Année:
1989
Type:
Article
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