Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand.
Southeast Asian J Trop Med Public Health
;
1999 Mar; 30(1): 161-5
Article
Dans Anglais
| IMSEAR
| ID: sea-35812
ABSTRACT
A virus isolate, ThCAr105/92, from a pool of mosquitos, Culex tritaeniorhynchus, collected in Chiang Mai, Thailand in 1992, appeared to be a member of the genus Flavivirus of the family Flaviviridae, based on the reverse transcription polymerase chain reaction (RT-PCR) using flavivirus cross-reacting primer pairs, electron microscopic examination, and serological tests. However, RT-PCR using Japanese encephalitis (JE) virus-specific primers showed that the isolate was different from JE virus. Sucrose density gradient sedimentation of the virus replicated in C6/36 cells indicated that the virus is relatively unstable in the infected culture fluids at 37 degrees C. Antibody prepared against this virus and a virus seed for the isolate were tested by cross neutralization against a panel of flaviviruses and the results showed that the new isolate was a distinct subtype of Tembusu virus.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Thaïlande
/
Virologie
/
Culture virale
/
ADN viral
/
Tests de neutralisation
/
Microscopie électronique
/
Sérotypie
/
RT-PCR
/
Flavivirus
/
Animaux
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Southeast Asian J Trop Med Public Health
Année:
1999
Type:
Article
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