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Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand.
Southeast Asian J Trop Med Public Health ; 1999 Mar; 30(1): 161-5
Article Dans Anglais | IMSEAR | ID: sea-35812
ABSTRACT
A virus isolate, ThCAr105/92, from a pool of mosquitos, Culex tritaeniorhynchus, collected in Chiang Mai, Thailand in 1992, appeared to be a member of the genus Flavivirus of the family Flaviviridae, based on the reverse transcription polymerase chain reaction (RT-PCR) using flavivirus cross-reacting primer pairs, electron microscopic examination, and serological tests. However, RT-PCR using Japanese encephalitis (JE) virus-specific primers showed that the isolate was different from JE virus. Sucrose density gradient sedimentation of the virus replicated in C6/36 cells indicated that the virus is relatively unstable in the infected culture fluids at 37 degrees C. Antibody prepared against this virus and a virus seed for the isolate were tested by cross neutralization against a panel of flaviviruses and the results showed that the new isolate was a distinct subtype of Tembusu virus.
Sujets)
Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Thaïlande / Virologie / Culture virale / ADN viral / Tests de neutralisation / Microscopie électronique / Sérotypie / RT-PCR / Flavivirus / Animaux Pays comme sujet: Asie langue: Anglais Texte intégral: Southeast Asian J Trop Med Public Health Année: 1999 Type: Article

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Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Thaïlande / Virologie / Culture virale / ADN viral / Tests de neutralisation / Microscopie électronique / Sérotypie / RT-PCR / Flavivirus / Animaux Pays comme sujet: Asie langue: Anglais Texte intégral: Southeast Asian J Trop Med Public Health Année: 1999 Type: Article