Establishment and characterization of a cholangiocarcinoma cell line from a Thai patient with intrahepatic bile duct cancer.
Asian Pac J Allergy Immunol
;
1991 Dec; 9(2): 153-7
Article
Dans Anglais
| IMSEAR
| ID: sea-36718
ABSTRACT
A new human cholangiocarcinoma cell line (HuCCA-1) was established from cholangiocarcinoma (CCA) tissue fragments surgically removed from a Thai patient with intrahepatic bile duct cancer. The growth medium used for the primary cell culture was Ham's F12 supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml epithelial growth factor (EGF). Approximately one month later, the cells were subcultured in Ham's F12 supplemented with only 10% FBS. The population doubling time was approximately 55 hr. Staining of the cells for cytokeratin and mucin confirmed that the cells were mucin-secreting tumor of epithelial cell origin. The supernatant fluid secreted a number of non-specific tumor markers including CA125 and traces of MCA and AFP. The ability of the HuCCA-1 cell line to synthesize specific marker that may have potential in the diagnosis of cholangiocarcinoma is now being investigated.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Thaïlande
/
Tumeurs des canaux biliaires
/
Humains
/
Mâle
/
Marqueurs biologiques tumoraux
/
Lignée cellulaire
/
Technique d'immunofluorescence
/
Cholangiome
/
Foie
/
Adulte d'âge moyen
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Asian Pac J Allergy Immunol
Année:
1991
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS