Induction of inflammatory cell accumulation using human mast cell tryptases.
Asian Pac J Allergy Immunol
;
2003 Sep; 21(3): 145-51
Article
Dans Anglais
| IMSEAR
| ID: sea-36811
ABSTRACT
The aim of this study was to investigate the effects of human tryptases on inflammatory cell accumulation in vivo. Various concentrations of purified lung or skin tryptases were injected into the peritoneum of BALB/c mouse. At 6 hours or 16 hours following injection, cells from the peritoneal lavage were collected and stained with modified Wright's stain. Differential cell counts were performed and results were expressed as absolute numbers of each cell type per mouse peritoneum. The results showed a dose-dependent infiltration of neutrophils with a maximal increase of up to 32 fold or 43 fold in numbers at 16 hours following an injection of skin and lung tryptases, respectively. Skin tryptase was able to attract more eosinophils than lung tryptase. Significant increases in lymphocyte and macrophage numbers were also observed. In conclusion, both skin and lung tryptases are able to induce nucleated cell accumulation in the peritoneum of mice with similar specificity and potency.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Péritoine
/
Peau
/
Mâle
/
Serine endopeptidases
/
Héparine
/
Lymphocytes
/
Différenciation cellulaire
/
Médiateurs de l'inflammation
/
Infiltration par les neutrophiles
/
Modèles animaux
Type d'étude:
Étude pronostique
langue:
Anglais
Texte intégral:
Asian Pac J Allergy Immunol
Année:
2003
Type:
Article
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