Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay.
Asian Pac J Allergy Immunol
;
2001 Mar; 19(1): 43-8
Article
Dans Anglais
| IMSEAR
| ID: sea-37225
ABSTRACT
A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count range of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 10(6) CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Thaïlande
/
Humains
/
ADN viral
/
Lymphocytes T CD4/
/
Infections à VIH
/
Réaction de polymérisation en chaîne
/
Sensibilité et spécificité
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
/
Numération des lymphocytes CD4
/
Détermination du point final
Type d'étude:
Etude diagnostique
/
Études d'évaluation
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Asian Pac J Allergy Immunol
Année:
2001
Type:
Article
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