Paternity testing by PCR-based STR analysis.
Article
Dans Anglais
| IMSEAR
| ID: sea-44071
ABSTRACT
We present application of polymerase chain reaction (PCR)-based short tandem repeat (STR) system for use in paternity testing. The process involves a single tube multiplex PCR of 9 STR loci on different chromosomes, in conjunction with Amelogenin sex test and internal size standards, followed by using an automated DNA sequencer to detect amplified products. The results showed that this system provided unambiguously reliable results. In addition, the method is useful for routine use in that it is robust and reproducible and provides a reliable means of paternity testing.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Paternité
/
Thaïlande
/
Humains
/
Mâle
/
Réaction de polymérisation en chaîne
/
Reproductibilité des résultats
/
Sensibilité et spécificité
/
Séquences répétées en tandem
Type d'étude:
Etude diagnostique
Pays comme sujet:
Asie
langue:
Anglais
Année:
2000
Type:
Article
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