Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization.
Indian J Med Microbiol
;
2007 Oct; 25(4): 391-4
Article
Dans Anglais
| IMSEAR
| ID: sea-53479
ABSTRACT
Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes specific for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly specific. Sensitivity testing showed that the probes were able to detect as low as 10 3 CFU/mL. In addition, FISH was carried out directly on positive blood culture samples and the detection of microorganisms took less than 2 h. We believe that FISH is a rapid method that can be used as a routine laboratory diagnostic technique for the detection of Acinetobacter spp. and Pseudomonas spp. in clinical samples.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Pseudomonas
/
Infections à Pseudomonas
/
Acinetobacter
/
Sang
/
Infections à Acinetobacter
/
Humains
/
Techniques bactériologiques
/
Sensibilité et spécificité
/
Bactériémie
/
Hybridation fluorescente in situ
Type d'étude:
Etude diagnostique
/
Études d'évaluation
langue:
Anglais
Texte intégral:
Indian J Med Microbiol
Thème du journal:
Microbiology
Année:
2007
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS