Detection of Enterobacteriaceae producing CTX-M extended spectrum beta-lactamases from a tertiary care hospital in south India.
Indian J Med Microbiol
;
2008 Apr-Jun; 26(2): 163-6
Article
Dans Anglais
| IMSEAR
| ID: sea-53526
ABSTRACT
A total of 23 clinical isolates (15 Escherichia coli and 8 Klebsiella pneumoniae), resistant to cefotaxime and ceftazidime recovered during 2002 and 2003, were investigated for production of CTX-M extended spectrum beta-lactamase (ESBL) by phenotypic and molecular methods. The presence of ESBL was tested by NCCLS phenotypic confirmatory test using cephalosporin/clavulanate combination discs and E-test ESBL strips. Determination of MIC of cefotaxime and ceftazidime was done with and without the presence of clavulanic acid by agar dilution technique. Polymerase chain reaction revealed the presence of CTX-M type ESBLs in 19 isolates. Further sequencing resulted in identification of CTX-M-15 ESBLs. This is the first report identifying CTX-M type ESBL from clinical isolates of E. coli and K. pneumoniae from a tertiary care hospital in south India.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Bêta-Lactamases
/
Humains
/
Infections à Klebsiella
/
ADN bactérien
/
Tests de sensibilité microbienne
/
Céphalosporines
/
Réaction de polymérisation en chaîne
/
Analyse de séquence d'ADN
/
Résistance aux bêta-lactamines
/
Escherichia coli
Type d'étude:
Etude diagnostique
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Indian J Med Microbiol
Thème du journal:
Microbiology
Année:
2008
Type:
Article
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