Development and evaluation of an in vitro isolation of street rabies virus in mouse neuroblastoma cells as compared to conventional tests used for diagnosis of rabies.
Indian J Med Microbiol
;
2007 Jul; 25(3): 263-6
Article
Dans Anglais
| IMSEAR
| ID: sea-53751
ABSTRACT
In vitro isolation of rabies virus using mouse neuroblastoma cells (MNA) was evaluated. The sensitivity and reliability of in vitro procedure was performed in comparison with mouse inoculation test (MIT), the in vivo method of virus isolation, direct fluorescent antibody test (FAT) and Sellers staining. Of the 33 animal brain samples tested, 24 (72.72%) were positive by MIT. Sensitivity of Sellers stain, FAT and rapid tissue culture infection test (RTCIT) was found to be 54.16, 100 and 91.6% respectively. Concordance of Sellers stain, FAT, RTCIT with MIT was found to be 66.6, 100 and 93.93% respectively. Two samples which were positive by FAT and MIT showed gross contamination in cell lines, which is one of the drawbacks of RTCIT. However, rabies virus could be isolated in MNA cells from two of the eight human cerebrospinal fluid (CSF) samples from clinico-epidemiologically suspected cases of rabies. Both MIT and FAT showed negative results in the two CSF samples. RTCIT appears to be a fast and reliable alternative to MIT and holds promise in antemortem diagnosis of rabies, which is otherwise, a challenging task for a reference laboratory.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Virus de la rage
/
Virologie
/
Humains
/
Bovins
/
Chats
/
Reproductibilité des résultats
/
Sensibilité et spécificité
/
Lignée cellulaire tumorale
/
Chiens
/
Animaux
Type d'étude:
Etude diagnostique
/
Études d'évaluation
langue:
Anglais
Texte intégral:
Indian J Med Microbiol
Thème du journal:
Microbiology
Année:
2007
Type:
Article
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