Existence of proviral porcine endogenous retrovirus in fresh and decellularised porcine tissues.
Indian J Med Microbiol
;
2008 Jul-Sep; 26(3): 228-32
Article
Dans Anglais
| IMSEAR
| ID: sea-53909
ABSTRACT
PURPOSE:
Swine are expected to be utilized as xenograft donors for both whole organ and cellular transplantation. A major concern in using porcine organs for transplantation is the potential of transmission of porcine endogenous retrovirus (PERV). Tissue-engineered or decellularised heart valves have already been implanted in humans and have been marketed by certain companies after Food and Drug Administration (FDA) approval. The aim of this study was to examine the existence of porcine endogenous retrovirus (PERV) in fresh and decellularised porcine tissues.METHODS:
Porcine tissues (both fresh and decellularised) were analysed using validated assays specific for PERV polymerase chain reaction (PCR), reverse transcriptase polymerase chain reaction (RT-PCR).RESULTS:
PERV specific GAG sequences were found in the porcine heart tissue samples using PCR for DNA and RT- PCR for RNA. All tissue samples (both fresh and treated tissues) like aortic valve, pulmonary valve and heart muscle showed the presence of PERV DNA. RT PCR for PERV was positive in all fresh tissues and was found to be negative in decellularised treated tissues.CONCLUSIONS:
PCR is a rapid, specific test for the detection of PERV virus in xenografts. These findings have demonstrated that the presence of proviral DNA form of PERV in porcine tissues needs to be carefully considered when the infectious disease potential of xenotransplantation is being assessed.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Transplantation hétérologue
/
Humains
/
Gènes gag
/
Réaction de polymérisation en chaîne
/
Provirus
/
Rétrovirus endogènes
/
RT-PCR
/
Ingénierie tissulaire
/
Animaux
langue:
Anglais
Texte intégral:
Indian J Med Microbiol
Thème du journal:
Microbiology
Année:
2008
Type:
Article
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