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Mini transposon vector mediated foreign gene expression in Mesorhizobium huakuii subsp. rengei.
Indian J Exp Biol ; 2004 Oct; 42(10): 1028-31
Article Dans Anglais | IMSEAR | ID: sea-55740
ABSTRACT
Among the transposable elements, mini-Tn5 transposon vector has proven to be of greater utility for insertion mutagenesis of variety of Gram negative bacteria. The mini-Tn5 vector containing promoter less egfp gene and gentamycin resistant gene was used for the present study. The transposon vector was introduced to M. huakuii from E. coli S17 by conjugation. The conjugants were screened for stable expression of egfp both in free-living and in nodules of Astragalus sinicus. The result showed that the conjugant #3 showed stable expression of green fluorescent both in free-living and bacteroid stage. The visualization of sym plasmid of wild strain and conjugants showed that conjugant #3 had a fragmentation of large sized plasmid into two but without affecting the nodulating ability. These results clearly indicated that mini-Tn5 vectors (Transposon vectors) the best alternate tools for plasmid vectors for integration of foreign genes in chromosomal DNA or symbiotic plasmid and expression, both in free-living and bacteroid stage of Rhizobium.
Sujets)
Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Protéines recombinantes / Éléments transposables d'ADN / Expression des gènes / Conjugaison génétique / Alphaproteobacteria / Astragalus / Protéines à fluorescence verte / Escherichia coli / Vecteurs génétiques langue: Anglais Texte intégral: Indian J Exp Biol Année: 2004 Type: Article

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Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Protéines recombinantes / Éléments transposables d'ADN / Expression des gènes / Conjugaison génétique / Alphaproteobacteria / Astragalus / Protéines à fluorescence verte / Escherichia coli / Vecteurs génétiques langue: Anglais Texte intégral: Indian J Exp Biol Année: 2004 Type: Article