Isolation of etiological agent of hydropericardium syndrome in chicken embryo liver cell culture and its serological characterization.
Indian J Exp Biol
; 2003 Aug; 41(8): 821-6
Article
de En
| IMSEAR
| ID: sea-56364
The virus causing hydropericardium syndrome was isolated in chicken embryo liver (CEL) cell culture from livers obtained from naturally infected broilers. The cytopathic effects characterized by rounding and degeneration of cells were visible 36 hr post infection in first passage. At 4th passage level, the infectivity titre was 5.24 log10 TCID50/ml. In May-Grunwald and Giemsa stained cells, basophilic intranuclear inclusions ('bird eye' inclusion), typical of aviadenovirus infection, were observed. The specificity of inclusion was confirmed by indirect immunofluorescence. Various serological tests, such as agar gel precipitation test, counter immuno electrophoresis, micro serum neutralization test and enzyme linked immunosorbent assay were also standardized to confirm the isolation of etiological agent of hydropericardium syndrome in CEL cell culture and to diagnose the disease in poultry.
Texte intégral:
1
Indice:
IMSEAR
Sujet Principal:
Épanchement péricardique
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Maladies de la volaille
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Tests sérologiques
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Embryon de poulet
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Infections à Adenoviridae
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Aviadenovirus
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Effet cytopathogène viral
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Corps d'inclusion viraux
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Animaux
Type d'étude:
Diagnostic_studies
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Etiology_studies
langue:
En
Texte intégral:
Indian J Exp Biol
Année:
2003
Type:
Article