Differential organization of a LINE-1 family in Indian pygmy field mice.
Indian J Exp Biol
;
2003 Jan; 41(1): 53-7
Article
Dans Anglais
| IMSEAR
| ID: sea-56571
ABSTRACT
Southern blot hybridization analysis of genomic DNAs digested with restriction endonuclease EcoR I and Ava II from Mus musculus domesticus, Mus booduga and Mus terricolor with a cloned repetitive DNA fragment of Mus booduga as a probe showed difference in restriction pattern of this DNA in these three species. Further Southern analysis of the BamH I digested genomic DNAs from these species hybridized with cloned DNA fragment as a probe and sequencing of the cloned DNA revealed that this 252 bp cloned DNA fragment is a part of BamHI repeat element of genus Mus and is 87% homologous to the contiguous portion of the Mus musculus domesticus LINE-1 element. The species specific fragment pattern generated by different restriction endonucleases using this DNA as a probe revealed difference in the organization of LINE-1 repetitive element in the three species of genus Mus.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
ADN
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Séquences répétées d'acides nucléiques
/
Similitude de séquences d'acides nucléiques
/
Clonage moléculaire
/
Éléments LINE
/
Animaux
/
Souris
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2003
Type:
Article
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