Protein A linked latex antisera test for detection of infectious flacherie of silkworm, Bombyx mori L. caused by B. mori infectious flacherie virus.
Indian J Exp Biol
;
1997 Nov; 35(11): 1203-7
Article
Dans Anglais
| IMSEAR
| ID: sea-56981
ABSTRACT
Protein A linked latex antisera (PALLAS) test was used for detection of B. mori infectious flacherie virus (BmIFV) in silkworms. The test was found superior to direct latex agglutination (DLA) test for detection of BmIFV in infected larval and faecal matter extracts and purified preparations. Latex beads sensitized with anti-BmIFV IgG were able to detect up to 75 ng/ml BmIFV in PALLAS test and 300 ng/ml in DLA test. BmIFV was detected in diluted extracts of infected larvae (up to 1200) and their faecal matter (up to 1500). BmIFV progressive infection was detected at 12 and 24 hr of post infection in PALLAS and DLA tests, respectively in both larval and faecal matter extracts. PALLAS tests provides a simple, specific and highly sensitive test for detection of infectious flacherie virus.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Protéine A staphylococcique
/
Tests au latex
/
Sérums immuns
/
Animaux
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1997
Type:
Article
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