Enhancement of haemopoietic stem cell adherence to bone-marrow derived stroma by phyto-haemagglutinin treated leucocyte conditioned medium (PHA-LCM).
Indian J Exp Biol
;
1994 Apr; 32(4): 225-8
Article
Dans Anglais
| IMSEAR
| ID: sea-56984
ABSTRACT
Stem cell adhesion to bone-marrow derived stroma, plays a crucial role in haemopoiesis. However, there is very little information as to the nature of the adhesion molecule. In this paper we have shown that human bone-marrow derived stroma can be established in tissue culture. This stroma is able to adhere human bone-marrow mononuclear cells including the multipotent stem cell, viz. CFU-GEMM. Their adherence increases when the stroma is treated with lymphokines in the form of PHA-treated leucocyte conditioned medium (PHA-LCM). Triton X-100 extracts of the untreated and PHA-LCM treated stroma were analysed on single dimension PAGE. It was observed that PHA-LCM treated stromal extracts showed two extra bands and an increase in the density of a band of approximately 14 kDa. Whether these changes have anything to do with the increased adhesion of stem cell is not yet known.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Phytohémagglutinine
/
Moelle osseuse
/
Humains
/
Cellules de la moelle osseuse
/
Cellules souches hématopoïétiques
/
Adhérence cellulaire
/
Cellules cultivées
/
Cellules stromales
/
Milieux de culture conditionnés
/
Leucocytes
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1994
Type:
Article
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