Identification of a 75 kDa highly immunodominant antigen from Mycobacterium smegmatis and cross-reactivity with other species.
Indian J Exp Biol
;
2001 Mar; 39(3): 255-62
Article
Dans Anglais
| IMSEAR
| ID: sea-57163
ABSTRACT
Three monospecific antibodies MSAb 1, MSAb 2 and MSAb 3 were raised in BALB/C mice against respective antigens. M. smegmatis whole cell lysate was first separated on SDS-PAGE and randomly chosen bands were cut and then used for immunization. Antibodies were collected as ascites by injecting mice with myeloma cell line P3X63 Ag 658.4. All the three antibodies showed high reactivity with denatured antigens compared to native. Different extent of cross-reactivity was observed as evident from ELISA. MSAb1 recognized a 75 kDa immunodominant antigen from M. smegmatis and 66 kDa from M. tuberculosis (H37Ra), respectively. An apparently similar molecular weight antigen shown to be present in M. tuberculosis (H37Ra) an avirulent strain and BCG, but not recognized by MSAb1. The 75 kDa antigen has a stimulatory effect on T-cell proliferation.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Spécificité d'espèce
/
Test ELISA
/
Lymphocytes T
/
Épitopes immunodominants
/
Mycobacterium smegmatis
/
Réactions croisées
/
Animaux
/
Souris
/
Masse moléculaire
/
Anticorps antibactériens
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2001
Type:
Article
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