Immunogenic activity of viral polypeptides of an Indian isolate of infectious bursal disease virus.
Indian J Exp Biol
; 1994 Oct; 32(10): 686-9
Article
de En
| IMSEAR
| ID: sea-58036
An Indian isolate of infectious bursal disease virus, i.e. IBDV-P/AD/81, was analysed for immunogenic activity of its structural polypeptides. Virus was purified from infected bursal homogenate by sucrose density gradient centrifugation. It showed five different structural polypeptides of 75.8, 45, 40.7, 33.1 and 27 kDa molecular weights in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Anti infectious bursal disease virus (IBDV) antibodies were tested using enzyme-linked immunosorbent assay (ELISA) and neutralization test (NT). Polypeptide 40.7 kDa (VP2) was known to have neutralizing epitopes. However, polyclonal anti VP2 failed to neutralize the virus. It was interpreted that VP2 had labile neutralizing epitopes which get altered confirmationally by SDS. Surprisingly, polyclonal anti 33.1 kDa (VP3) had mild neutralizing activity.
Texte intégral:
1
Indice:
IMSEAR
Sujet Principal:
Peptides
/
Protéines virales
/
Poulets
/
Virus de la bursite infectieuse
/
Inde
/
Animaux
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Indian J Exp Biol
Année:
1994
Type:
Article