Lytic effect of Bacillus subtilis elastase on gram-positive and negative bacteria.
Indian J Exp Biol
;
1992 May; 30(5): 380-3
Article
Dans Anglais
| IMSEAR
| ID: sea-58536
ABSTRACT
Elastase of B. subtilis 6a caused lysis of freshly grown cells of Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, Salmonella typhi and Pseudomonas aeruginosa and Gram-positive (B. subtilis) bacteria. Heat killed and lyophilised Gram-positive and negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Bacillus subtilis
/
Protéines bactériennes
/
Bactériolyse
/
Pancreatic elastase
/
Bactéries à Gram négatif
/
Bactéries à Gram positif
/
Concentration en ions d'hydrogène
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1992
Type:
Article
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