Comparison of two colorimetric assays to determine viral infectivity in micro culture virus titration.
Indian J Exp Biol
;
1999 Dec; 37(12): 1223-6
Article
Dans Anglais
| IMSEAR
| ID: sea-58690
ABSTRACT
Efficacy of two colorimetric assays, viz. MTT (3-4,5-dimethylthiazol-2-(yl-2,5-diphenyl tetrazolium bromide) and neutral red (NR) assays, performed by integrating them to micro culture virus titration (MCVT), was compared with the conventional MCVT method in terms of percentages of infectivity and 50% infectivity end points by employing Polio virus type-3 and Dengue virus type 4 as the candidate viruses. The results suggested that MTT assay has an edge over NR assay as well as conventional MCVT method. For the first time, NR assay has been successfully employed for the determination of virus infectivity titre.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Sels de tétrazolium
/
Thiazoles
/
Virologie
/
Cellules Vero
/
Humains
/
Lignée cellulaire
/
Chlorocebus aethiops
/
Colorimétrie
/
Effet cytopathogène viral
/
Poliovirus
Type d'étude:
Etude diagnostique
/
Études d'évaluation
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1999
Type:
Article
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