A protocol for shoot multiplication from foliar meristem of Vanda spathulata (L.) Spreng.
Indian J Exp Biol
;
2003 Aug; 41(8): 924-7
Article
Dans Anglais
| IMSEAR
| ID: sea-58761
ABSTRACT
Leaf explants collected from flowering plants of Vanda spathulata were cultured in Mitra medium with combinations of 6-benzyladenine (BA; 13.2-88.8 microM) and indole-3-acetic acid (IAA; 0.0 -85.6 microM). Combination of BA (66.6 microM) and IAA (28.5 microM) induced maximum shoots (17.33) from foliar meristems (leaf base). BA individually did not induce caulogenesis in leaf explants. For optimized multiplication, BAIAA (21 microM) was essential at 22.2- 88.8 microM of BA. Re-cultured leaf explants produced lesser number of shoots compared to original explants and were nearly equal at combinations of 22.2-44.4 microM of BA and 5.7-28.5 microM of IAA. Rooting of shoots (> 95%) occurred in medium containing banana pulp (75 gl(-1)) and IAA (5.7 microM) within 3-9 weeks. Plantlets with 2-5 roots of 2-5 cm length established easily in community pots at 80-90% rates without hardening.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Adénine
/
Feuilles de plante
/
Méristème
/
Milieux de culture
/
Orchidaceae
/
Acides indolacétiques
/
Kinétine
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2003
Type:
Article
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