Angiotensin II binding to human embryonic lung fibroblasts causes transient changes in cytosolic calcium and promotes fibrin gel contraction.
Indian J Exp Biol
;
1994 Sep; 32(9): 603-6
Article
Dans Anglais
| IMSEAR
| ID: sea-59126
ABSTRACT
Contraction of granulation tissue is promoted by the peptide vasoconstrictor angiotensin II (AII), which presumably acts on the fibroblasts present in granulation tissue. Direct effects of AII on fibroblast contraction were examined using an in vitro fibrin gel contraction model. Fibrin gels were formed by mixing cultured human embryonic lung fibroblasts with fibrinogen, in the presence of thrombin and measurement of gel volume was used to determine the extent of fibrin gel contraction by fibroblasts. AII stimulated an additional 28.5 +/- 3% decrease in gel size after 24 hr, AII is also shown to specifically bind to human embryonic lung fibroblasts and stimulate rapid transient increases in cytosolic calcium. AII stimulates contraction of human embryonic lung fibroblasts as reflected by acceleration of fibrin gel contraction. Fibrin clot contraction contributes to extra cellular matrix reorganization during initial stages of wound repair and AII-stimulated fibroblast contraction may accelerate this process.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Liaison aux protéines
/
Facteurs temps
/
Humains
/
Fibrine
/
Angiotensine-II
/
Cellules cultivées
/
Calcium
/
Cytosol
/
Fibroblastes
/
Gels
Type d'étude:
Etude d'étiologie
/
Étude pronostique
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1994
Type:
Article
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