Binary conjugational transfer system of vectors pRK290 and pLAFRI is proficient both ways between Escherichia coli and Rhizobium.
Indian J Exp Biol
;
1990 Oct; 28(10): 994-5
Article
Dans Anglais
| IMSEAR
| ID: sea-59151
ABSTRACT
Wide host range vector plasmids pRK290 and pLAFRI carrying genomic fragments of Rhizobium are transferable both ways between R. meliloti and R. leguminosarum cells on the one hand and to E. coli cells on the other, in triparental matings involving E. coli cells carrying pRK2013, the helper for Tra functions to the vector plasmids. The vector plasmids pRK290 and pLAFRI can be employed for recovering clones harbored by R. leguminosarum and R. meliloti by transfer to Rhizobium cells by direct matings of the library with them.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Plasmides
/
Rhizobium
/
Clonage moléculaire
/
Conjugaison génétique
/
Escherichia coli
/
Vecteurs génétiques
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1990
Type:
Article
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