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In vitro capacitation of goat spermatozoa.
Indian J Exp Biol ; 1989 Nov; 27(11): 921-4
Article Dans Anglais | IMSEAR | ID: sea-59788
ABSTRACT
Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement which was needed for motility maintenance/activation and for the initiation of acrosome reaction. A 2-fold increase in cAMP content was measured over 3 hr period of incubation of goat epididymal spermatozoa which was not seen when calcium ions were either omitted or chelated with EGTA. There is thus a definite involvement of Ca2+ ions and cAMP in capacitation and acrosome reaction of goat spermatozoa.
Sujets)
Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Capacitation des spermatozoïdes / Mobilité des spermatozoïdes / Spermatozoïdes / Acrosome / Mâle / Capra / Calcium / AMP cyclique / Animaux langue: Anglais Texte intégral: Indian J Exp Biol Année: 1989 Type: Article

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Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Capacitation des spermatozoïdes / Mobilité des spermatozoïdes / Spermatozoïdes / Acrosome / Mâle / Capra / Calcium / AMP cyclique / Animaux langue: Anglais Texte intégral: Indian J Exp Biol Année: 1989 Type: Article