In vitro capacitation of goat spermatozoa.
Indian J Exp Biol
;
1989 Nov; 27(11): 921-4
Article
Dans Anglais
| IMSEAR
| ID: sea-59788
ABSTRACT
Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement which was needed for motility maintenance/activation and for the initiation of acrosome reaction. A 2-fold increase in cAMP content was measured over 3 hr period of incubation of goat epididymal spermatozoa which was not seen when calcium ions were either omitted or chelated with EGTA. There is thus a definite involvement of Ca2+ ions and cAMP in capacitation and acrosome reaction of goat spermatozoa.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Capacitation des spermatozoïdes
/
Mobilité des spermatozoïdes
/
Spermatozoïdes
/
Acrosome
/
Mâle
/
Capra
/
Calcium
/
AMP cyclique
/
Animaux
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1989
Type:
Article
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