Detergent-mediated destaining of coomassie brilliant blue-stained SDS polyacrylamide gels.
Indian J Exp Biol
;
2001 Jan; 39(1): 95-7
Article
Dans Anglais
| IMSEAR
| ID: sea-60278
ABSTRACT
A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-mediated destaining procedure, the present method is economical and user-friendly.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Magenta I
/
Détergents
/
Électrophorèse sur gel de polyacrylamide
/
Indicateurs et réactifs
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2001
Type:
Article
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