In vitro high frequency regeneration of plantlets of Vigna mungo and their ex vitro growth.
Indian J Exp Biol
;
2001 Sep; 39(9): 916-20
Article
Dans Anglais
| IMSEAR
| ID: sea-60528
ABSTRACT
Of the five explants of V. mungo var. T9 used, the excised shoot tips gave best response with regard to offshoot formation followed by the embryonal axis explants. While a treatment comprising 0.5 mgL(-1) BAP, 0.5 mgL(-1) 2iP and 0.1 mgL(-1) NAA induced differentiation of an average 10 offshoots in shoot tip explants, only 3 offshoots were formed in the explants of embryonal axis in a treatment containing 0.5 mgL(-1) BAP and 0.1 mgL(-1) NAA, found optimum for them. Multiple shoots differentiated when explants with earlier regenerated and growing offshoots were first cultured in a treatment containing 0.1 mgL(-1) BAP, 0.25 mgL(-1) IAA and 5 mgL(-1) CCC and then subcultured in the same treatment but having only 1 mgL(-1) CCC. The isolated shoots rooted in 0.5 mgL(-1) IAA resulted in the formation of complete plantlets of an average height of 15 cm in 20 days. The in vitro-regenerated plants grew normally under field conditions and came to flowering as well.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Facteur de croissance végétal
/
Régénération
/
Pousses de plante
/
Milieux de culture
/
Techniques de culture
/
Fabaceae
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2001
Type:
Article
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