Production of fungal cell wall degrading enzymes by a biocontrol strain of Bacillus subtilis AF 1.
Indian J Exp Biol
;
2005 Oct; 43(10): 892-6
Article
Dans Anglais
| IMSEAR
| ID: sea-60601
ABSTRACT
Fungal cell wall degrading chitinases and glucanases attained significance in agriculture, medicine, and environment management. The present study was conducted to describe the optimum conditions required for the production of beta-1,4-N-acetyl glucosaminidase (NAGase) and beta-1,3-glucanase by a biocontrol strain of Bacillus subtilis AF 1. B. subtilis AF 1 was grown in minimal medium with colloidal chitin (3.0%) and yeast extract (0.3% YE ) and incubated at pH 7.0 and 30 degrees C on constant shaker at 180 rpm for 6 days produced highest amounts of NAGase. Presence of 0.5 mM of phenyl methyl sulfonyl fluoride (PMSF) and 0.04% of Tween 20 further improved the enzyme production. B. subtilis AF 1 grown in minimal medium with laminarin (1%) and yeast extract (0.3%) for 3 days produced maximum amount of beta-1,3-glucanase. These conditions can be further scaled-up for large-scale production of NAGase and beta-1,3-glucanase by B. subtilis AF 1.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Polyosides
/
Polysorbates
/
Acetylglucosaminidase
/
Température
/
Bacillus subtilis
/
Facteurs temps
/
Composés tosyliques
/
Carbone
/
Paroi cellulaire
/
Milieux de culture
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2005
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS