Genetic transformation of Robinia pseudoacacia by Agrobacterium tumefaciens.
Indian J Exp Biol
;
2003 Feb; 41(2): 149-53
Article
Dans Anglais
| IMSEAR
| ID: sea-60697
ABSTRACT
Transgenic Robinia pseudoacacia plants were obtained by Agrobacterium tumefaciens mediated gene transfer. Agrobacterium strain LBA4404 harbouring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and beta-glucuronidase (GUS) genes was co-cultivated with hypocotyl segments of in vitro raised seedlings of Robinia. Parameters important for high efficiency regeneration and transformation rates included type of explant, pre-conditioning of explants and appropriate length of co-cultivation period with Agrobacterium. A transformation frequency 16.67% was obtained by 48 hr of pre-conditioning followed by 48 hr of co-cultivation. Transformed tissue was selected by the ability to grow on kanamycin containing medium. Successful regeneration was followed after histochemical GUS assay for the detection of transgenic tissue. This transformation procedure has the potential to expand the range of genetic variation in Robinia.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Plasmides
/
Transformation génétique
/
Protéines de fusion recombinantes
/
Végétaux génétiquement modifiés
/
Agrobacterium tumefaciens
/
Transgènes
/
Kanamycin kinase
/
Robinia
/
Plant
/
Glucuronidase
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2003
Type:
Article
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