Attachment of spermatozoa to in vitro matured bovine oocytes in TCM-199 and MEM media.
Indian J Exp Biol
;
1998 Apr; 36(4): 367-70
Article
Dans Anglais
| IMSEAR
| ID: sea-60989
ABSTRACT
Investigation was undertaken to study the efficacy of TCM 199 and MEM media in the process of oocyte maturation and fertilization after harvesting the oocytes from slaughtered cow and heifer ovaries by aspiration and mincing techniques. Out of 354 number of ovaries, mincing method could yield 1.35 (50.98%) of good quality oocytes per ovary as comparison to 0.61 (55.45%) by aspiration method. The TCM 199 and MEM could record the maturation of 85.71% and 60.26% of oocytes and the rate of sperm penetration was higher in TCM 199 matured oocytes (97.21%) as compared to MEM matured ones (74.46%). The staining of oocytes by aceto-orcein stain was also employed.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Ovocytes
/
Interaction sperme-ovule
/
Spermatozoïdes
/
Femelle
/
Mâle
/
Bovins
/
Fécondation in vitro
/
Milieux de culture
/
Animaux
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1998
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS