Effect of C-terminal 44 amino acids deletion on activity of Haemophilus influenzae UvrA protein.
Indian J Exp Biol
;
2006 Jan; 44(1): 7-13
Article
Dans Anglais
| IMSEAR
| ID: sea-61164
ABSTRACT
UV-sensitive mutant strain of Haemophilus influenzae Rd MBH3, is 20 times more sensitive to UV irradiation than the wild type strain. The mutation responsible for increased UV sensitivity of the strain was identified as G --> A transition predicting synthesis of truncated UvrAdeltaC44 protein (Balsara & Joshi). Recombinant UvrAdeltaC44 protein was purified for the first time under denaturing conditions. The molecular weight of the recombinant protein was estimated as approximately100 kDa. Recombinant UvrAdeltaC44 protein was found to be less efficient in its ATPase and DNA binding activity as compared to the wild type protein. Recombinant plasmid carrying uvrAdeltaC44 gene could partially complement the UvrA deficiency in E. coli UvrA mutant.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Radiotolérance
/
Protéines bactériennes
/
Rayons ultraviolets
/
Protéines recombinantes
/
ADN bactérien
/
Séquence nucléotidique
/
Haemophilus influenzae
/
Clonage moléculaire
/
Délétion de séquence
/
Adenosine triphosphatases
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2006
Type:
Article
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