Detection of Theileria annulata carrier cattle by PCR.
Indian J Exp Biol
;
2000 Mar; 38(3): 283-4
Article
Dans Anglais
| IMSEAR
| ID: sea-61248
ABSTRACT
A simple method for treating bovine blood samples for direct detection of T. annulata in carriers, after polymerase chain reaction (PCR) amplification of small subunit ribosomal RNA (SSU rRNA) gene is described. The threshold of detection of the PCR-assay was an erythrocytic parasitaemia of 0.00008% corresponding to 16 infected bovine erythrocytes. In 50 known carriers, 42 were positive in PCR, in which 8 cattle revealed presence of T. annulata in stained blood smear under microscope.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Theilériose
/
Bovins
/
Séquence nucléotidique
/
État de porteur sain
/
Réaction de polymérisation en chaîne
/
Theileria annulata
/
Gènes de protozoaire
/
Amorces ADN
/
Animaux
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2000
Type:
Article
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