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Determination of IAA in brassinolide treated coleoptiles of wheat by a modified indirect ELISA with polyclonal antibodies.
Indian J Exp Biol ; 1996 Mar; 34(3): 257-61
Article Dans Anglais | IMSEAR | ID: sea-61266
ABSTRACT
An indirect enzyme immunoassay for detection of as little as 10-50 pmole IAA is described for the first time. The assay is based on the development of highly specific polyclonal antibodies against the carboxyl site of IAA. The binding specificity is nearly as high as the radioimmunoassay and the titre of the specific antibody was also remarkably high (140,000 of the primary antibody). Such an easy, rapid, specific and highly sensitive assay would be extremely useful in gaining more information on the mode of action of phytohormones, and their effects on physiological processes.
Sujets)
Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Facteur de croissance végétal / Lapins / Stéroïdes hétérocycliques / Triticum / Test ELISA / Cholestanols / Cotylédon / Acides indolacétiques / Animaux / Anticorps langue: Anglais Texte intégral: Indian J Exp Biol Année: 1996 Type: Article

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Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Facteur de croissance végétal / Lapins / Stéroïdes hétérocycliques / Triticum / Test ELISA / Cholestanols / Cotylédon / Acides indolacétiques / Animaux / Anticorps langue: Anglais Texte intégral: Indian J Exp Biol Année: 1996 Type: Article