A rapid method for the preparation of plasmid DNA.
Indian J Exp Biol
;
1995 May; 33(5): 387-91
Article
Dans Anglais
| IMSEAR
| ID: sea-61738
ABSTRACT
A rapid method was developed to prepare plasmid DNA to be used for in vitro transcription, subcloning and sequencing. Clean plasmid DNA can be prepared within 90 min using this method and the quality of the plasmid DNA was found to be suitable for sequencing, preparation of in vitro RNA transcript and electroporation. Using this method plasmid DNA can be prepared from 2 ml to 100 ml culture. The yield of the recombinant plasmids was found to be in the range of 50-100 micrograms from 10 ml culture depending on the low and high copy number plasmids. The advantage of this method is that one can easily and rapidly prepare large quantities of plasmid DNA of high quality to be used for various purposes.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Plasmides
/
Facteurs temps
/
ADN
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1995
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS