Sperm-mediated gene transfer into oocytes of the golden hamster: assessment of sperm function.
Indian J Exp Biol
;
1999 Nov; 37(11): 1085-92
Article
Dans Anglais
| IMSEAR
| ID: sea-61830
ABSTRACT
The possibility of sperm as a vehicle to deliver foreign DNA to oocytes was tested in hamsters. Epididymal spermatozoa, incubated with linearized plasmid DNA encoding ovine growth hormone (pCMXoGH), showed a spontaneous tendency to interact with DNA. Kinetics of sperm uptake of DNA was determined by using [32P]-labeled DNA. Spermatozoa took up the added DNA by 15-30 min and the uptake was inhibited by human seminal fluid in a dose dependent manner. Addition of DNA did not affect the functional competence of spermatozoa, in terms of their ability to undergo capacitation and acrosome reaction (34.5% +/- 2.2 vs 35% +/- 1.5). The fertilizing ability of DNA treated-spermatozoa from hamsters and humans was assessed by zona-free hamster egg penetration assay. Number of sperm penetrated per oocyte were 23 +/- 4.5 and 1.4 +/- 1.3 for hamster and human spermatozoa, respectively. Penetrated oocytes harbored sperm-treated DNA both with hamster (30.2 cpm/oocyte) and human (19.2 cpm/oocyte) spermatozoa. These results show that the hamster and human spermatozoa have a strong tendency to interact with exogenous (foreign) DNA and are able to transfer DNA to oocytes. Sperm may be used as a vector for DNA transfer and this approach has potential in the production of transgenic animals.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Interaction sperme-ovule
/
Spermatozoïdes
/
Femelle
/
Humains
/
Mâle
/
Séquence nucléotidique
/
Cricetinae
/
Mesocricetus
/
Techniques de transfert de gènes
/
Amorces ADN
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1999
Type:
Article
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