Immunoelectrophoresis of mycobacterial antigens.
Indian J Exp Biol
;
1990 Sep; 28(9): 812-5
Article
Dans Anglais
| IMSEAR
| ID: sea-62741
ABSTRACT
Polyvalent antiserum to culture filtrate of H37 Ra M. tuberculosis was raised in rabbits. Monospecific antiserum was raised against M. tuberculosis antigen-5, prepared from the culture filtrates by immunoabsorbent affinity chromatography. On immunoelectrophoresis, antigen-5 demonstrated single precipitin arc against polyvalent and monospecific antisera. The culture filtrate antigen demonstrated multiple precipitin arcs against polyvalent antiserum and single precipitin are against monospecific antiserum. Antigen-5 could be isolated and characterized from the culture filtrate of H37 Ra M. tuberculosis. Immunoelectrophoresis could be one of the method to characterize the mycobacterial antigens prepared in the laboratory.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Lapins
/
Tuberculine
/
Mâle
/
Immunoélectrophorèse
/
Animaux
/
Mycobacterium tuberculosis
/
Antigènes bactériens
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
1990
Type:
Article
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