Ultrastructural studies on nodules induced by pyrimidine auxotrophs of Sinorhizobium meliloti.
Indian J Exp Biol
;
2001 Apr; 39(4): 371-7
Article
Dans Anglais
| IMSEAR
| ID: sea-62902
ABSTRACT
Twenty three pyrimidine auxotrophs of Sinorhizobium meliloti Rmd201 were generated by random mutagenesis with transposon Tn5. On the basis of biochemical characters these auxotrophic mutants were classified into car, pyrC and pyrE/pyrF categories. All auxotrophs induced white nodules which were ineffective in nitrogen fixation. Light and electron microscopic studies revealed that the nodules induced by pyrC mutants were more developed than the nodules of car mutants. Similarly the nodules induced by pyrE/pyrF mutants had more advanced structural features than the nodules of pyrC mutants. The nodule development in case of pyrE/pyrF mutants was not to the extent observed in the parental strain. These results indicated that some of the intermediates and/or enzymes of pyrimidine biosynthetic pathway of S. meliloti play a key role in bacteroidal transformation and nodule development.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Pyrimidines
/
Symbiose
/
Microscopie électronique
/
Mutagenèse
/
Sinorhizobium meliloti
/
Racines de plante
/
Medicago sativa
/
Fixation de l'azote
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2001
Type:
Article
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