A simple method for efficient extraction and purification of C-phycocyanin from Spirulina platensis Geitler.
Indian J Exp Biol
;
2005 Mar; 43(3): 277-9
Article
Dans Anglais
| IMSEAR
| ID: sea-63141
ABSTRACT
Phycocyanin is a major light harvesting accessory pigment of red algae and cyanobacteria. In the light of its many commercial applications in food and pharmaceutical industry, purity of the pigment plays a major role. Pharmaceutical industry demands a highly pure phycocyanin with A620/280 ratio of 4 and food industry a ratio of 2. In the present study phycocyanin was extracted in sodium phosphate buffer (pH 7) after macerating in liquid nitrogen. The crude phycocyanin thus extracted was precipitated with 50% ammonium sulphate, purified by dialysis and finally by gel filtration chromatography. Pure phycocyanin was finally obtained with an A620/A280 value of 4.98.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Phycocyanine
/
Spectrophotométrie
/
Protéines bactériennes
/
Biochimie
/
Extraits de plantes
/
Chromatographie sur gel
/
Cyanobactéries
/
Rhodophyta
/
Électrophorèse sur gel de polyacrylamide
/
Spirulina
langue:
Anglais
Texte intégral:
Indian J Exp Biol
Année:
2005
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS