Characteristic analysis of fibracel carriers for animal cell culture / 中国生物制品学杂志

ABSTRACT

@#Objective To investigate the surface properties of different fibracel carriers and their culture effects on different cells.Methods Three fibracel carriers(A,B,C)were selected to analyze the chemical element composition of their materials,and the contact angles of the carriers before and after pretreatment with 0. 1 mol/L NaOH solution were tested. By measuring the adhesion effect and glucose consumption of Vero,MDCK and MRC-5 cells on the carrier,and observing the cell growth state by fluorescent staining,the cell adhesion efficiency and culture effect of the three carriers were compared and analyzed.Results The three carriers were mainly composed of C,H,O,and contained a small amount of N and S elements. Before pretreatment,the contact angle of carrier B was 0°,which was significantly lower than that of A[(109 ± 3. 13)°]and C[(121 ± 6. 82)°]（each F = 709. 1,each P < 0. 000 1）,and the hydrophilicity was stronger. Carriers A and C had poor hydrophilicity. After pretreatment,the contact angles of the surfaces of the three carriers A,B,and C were all 0°,with no significant difference(F = 0. 069 4,P > 0. 05),all of which were hydrophilic. The adherence rates of the three types of carriers within 3 h of cell culture were all above 80%. The cells were dense and evenly grown on the carrier fibers,the glucose consumption curves tended to“S”type,and the continuous cell culture effect was good. The total glucose consumption of carrier A and carrier C was basically the same,and carrier B was lower than carrier A and carrier C.Conclusion The chemical element composition and the relationship between the hydrophilic and hydrophobic properties of the three fibracel carriers were analyzed,and the adhesion rate and culture effect of Vero,MDCK and MRC-5 cells were evaluated,which provide reference for the subsequent research and production application of fibracel carriers.