Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases / 法医学杂志
Journal of Forensic Medicine
;
(6): 452-456, 2023.
Article
Dans Anglais
| WPRIM
| ID: wpr-1009377
ABSTRACT
OBJECTIVES@#To establish a simple and rapid qualitative and quantitative detection method of dexmedetomidine in blood.@*METHODS@#Blood was separated on the Allure PFP Propyl liquid chromatography column with isocratic elution after it was precipitated by acetonitrile and filtered. Qualitative and quantitative analysis of dexmedetomidine was performed using positive ion scan mode and multi-reaction monitoring mode.@*RESULTS@#The limit of detection of dexmedetomidine in blood was 0.2 ng/mL and the limit of quantification was 0.5 ng/mL. The linearity of the method was good in the range of 0.5-1 000 ng/mL, and the correlation coefficient was greater than 0.99. The accuracy of the method was 90.34%-112.67% and the extraction recovery was 50.05%-91.08%, with no significant matrix effect.@*CONCLUSIONS@#This method is simple, selective and suitable for the qualitative and quantitative analysis of dexmedetomidine in blood, which can provide a reference for drug-facilitated cases involving dexmedetomidine.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Chromatographie en phase liquide
/
Dexmédétomidine
/
Spectrométrie de masse en tandem
langue:
Anglais
Texte intégral:
Journal of Forensic Medicine
Année:
2023
Type:
Article
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