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Functional characterization of CYP81C16 involved in the tanshinone biosynthetic pathway in Salvia miltiorrhiza / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.) ; (6): 938-949, 2023.
Article Dans Anglais | WPRIM | ID: wpr-1011005
ABSTRACT
Danshen, the dried roots and rhizomes of Salvia miltiorrhiza Bunge (S. miltiorrhiza), is widely used in the treatment of cardiovascular and cerebrovascular diseases. Tanshinones, the bioactive compounds from Danshen, exhibit a wide spectrum of pharmacological properties, suggesting their potential for future therapeutic applications. Tanshinone biosynthesis is a complex process involving at least six P450 enzymes that have been identified and characterized, most of which belong to the CYP76 and CYP71 families. In this study, CYP81C16, a member of the CYP71 clan, was identified in S. miltiorrhiza. An in vitro assay revealed that it could catalyze the hydroxylation of four para-quinone-type tanshinones, namely neocryptotanshinone, deoxyneocryptotanshinone, and danshenxinkuns A and B. SmCYP81C16 emerged as a potential broad-spectrum oxidase targeting the C-18 position of para-quinone-type tanshinones with an impressive relative conversion rate exceeding 90%. Kinetic evaluations andin vivo assays underscored its highest affinity towards neocryptotanshinone among the tested substrates. The overexpression of SmCYP81C16 promoted the accumulation of (iso)tanshinone in hairy root lines. The characterization of SmCYP81C16 in this study accentuates its potential as a pivotal tool in the biotechnological production of tanshinones, either through microbial or plant metabolic engineering.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Quinones / Racines de plante / Régulation de l'expression des gènes végétaux / Salvia miltiorrhiza / Voies de biosynthèse Limites du sujet: Humains langue: Anglais Texte intégral: Chinese Journal of Natural Medicines (English Ed.) Année: 2023 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Quinones / Racines de plante / Régulation de l'expression des gènes végétaux / Salvia miltiorrhiza / Voies de biosynthèse Limites du sujet: Humains langue: Anglais Texte intégral: Chinese Journal of Natural Medicines (English Ed.) Année: 2023 Type: Article