The inhibitory effect of curcumin on B16F10 cells and its mechanism / 中国药理学通报

ABSTRACT

Aim To investigate the effect of curcumin extract on melanin production and melanosome transport, and to explore the possible mechanism of the curcumin extract on microenvironment. Methods (1) B16F10 and HaCaT cells were cultured with different concentrations of curcumin. The proliferation ability was detected by MTT method. Melanin synthesis and tyrosinase activity in B16F10 cells were detected by NaOH pyrolysis method and Oxidation dopamine response in vitro. The expression levels of key proteins were detected by Western blot. (2) B16F10 cells were cultured with different concentrations of ISG15 protein. NaOH pyrolysis method and Oxidation dopamine response in vitro were used to detect melanin synthesis and tyrosinase activity in B16F10 cells. Results Curcumin could directly inhibited tyrosinase activity and melanin production, and inhibit melanocyte migration within a certain concentration range. ISG15 protein could enhance the melanin production, tyrosinase activity. Curcumin could reduce the expression of the ISG15 in HaCaT cell, change the microenvironment of melanocyte, and indirectly inhibit melanin synthesis through ISG15. Conclusions In addition to directly inhibiting melanin synthesis, curcumin can also play an indirect role in inhibiting melanin synthesis by inhibiting the expression of ISG15 protein and altering the microenvironment of melanocytes.