Establishment and Application of Visual LAMP Methods for QuickDetection of Vibrio Splendidus in Aquatic Products and Water / 中国生物化学与分子生物学报
Chinese Journal of Biochemistry and Molecular Biology
;
(12): 108-119, 2022.
Article
Dans Chinois
| WPRIM
| ID: wpr-1015744
ABSTRACT
A visualized, rapid, simple method was developed based on loop-mediated isothermal amplification (LAMP) to detect Vibrio splendidus in aquatic products and aquaculture waters. TakingVibrio splendidus as the research object, and the toxR gene of Vibrio splendidus as the target gene, the boiling method was determined as a fast method suitable for the genomic DNA extraction of Vibriosplendidus. The optimized and screened primers could specifically detect Vibrio splendidus, and the sensitivity of nucleic acid concentration detection could reach 10
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Biochemistry and Molecular Biology
Année:
2022
Type:
Article
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