Clinical significance of NFAT5 expression in hepatocellular carcinoma and its regulatory effect on the expression of inflammatory related factors IL-6 and TNF-α / 中华内分泌外科杂志

ABSTRACT

Objective:To explore the clinical significance of nuclear factor of activated T cells 5 (NFAT5) expression in hepatocellular carcinoma and its regulatory effect on the expression of inflammatory related factors IL-6 and TNF-α.Methods:Immunohistochemistry was used to detect the expression of NFAT5 in the tissues of 76 patients with liver cancer and their adjacent tissues. HuH-7 cells were divided into experimental group and control group. The cells in the experimental group were transfected with NFAT5-siRNA plasmid, and the cells in the control group were transfected with NC-siRNA plasmid. The content of NFAT5 mRNA in the cells of each group was detected by fluorescence quantitative PCR, and the content of NFAT5 mRNA in each group was detected by Western blotting. The expression of NFAT5, IL6 and TNF-α in the cells, and the proliferation ability of the cells in each group was detected by CCK8.Results:The positive rate of NFAT5 in liver cancer tissues was 81.58% (62/76) , and the expression rate in adjacent tissues was 6.58% (5/76) . The expression rate of NFAT5 in hepatocellular carcinoma tissues was significantly higher than that in adjacent tissues, and the difference was statistically significant. Academic significance ( P<0.001) . After transfection of siRNA, the expression levels of NFAT5 mRNA in HuH-7 cells in experimental group and control group were 1.03±0.19 and 0.33±0.11, respectively. The protein expression levels of NFAT5 were 0.94±0.14 and 0.26±0.07, respectively, and the expression levels of IL6 were 1.09±0.16 and 0.49±0.10, respectively. The expression levels of TNF-α were 1.21±0.13 and 0.28±0.08, respectively, and the expressions of IL6 and TNF-α were significantly decreased (all P<0.05) . The absorbance of HuH-7 cells in the experimental group and the control group at 120 h was 2.21±0.12 and 1.12±0.11, respectively, and the cell proliferation ability of the experimental group was significantly decreased ( P<0.05) . Conclusion:The expression of NFAT5 is significantly increased in hepatocellular carcinoma, which may be related to the malignancy of hepatocellular carcinoma. And NFAT5 affects the proliferation of hepatocellular carcinoma cells by regulating the expression of IL6 and TNF-α.