Effects of acupuncture on the lung tissue of asthma model rats of expression levels of α-smooth muscle actin and fibronectin / 中国中西医结合急救杂志

ABSTRACT

Objective To observe the effect of acupuncture on lung tissue of asthma model rats ofα-smooth muscle actin(α-SMA),fibronectin(FN)expression levels.Methods A total of 40 male SPF Sprague-Dawley(SD)rats were randomly divided into normal control group,model group,drug group and acupuncture group,with 10 rats in each group.On the 1st and 8th days of the experiment,the normal control group was intraperitoneally injected with 1 mL of 0.9％ sodium chloride solution,and the model group was intraperitoneally injected with 1 mL of 10％ compound ovalbumin solution to sensitize.On the 15th day of the experiment,the normal control group was nebulized with 0.9％ sodium chloride solution for 20 minutes,and the model group was nebulized with 1％ ovalbumin solution for 20 minutes to stimulate asthma.After successful modeling,the normal control group and the model group were not treated,and the drug group was intraperitoneally injected with 0.05％ dexamethasone sodium phosphate solution,once a day,for 10 days.In the acupuncture group,Feishu,Dazhui and Fengmen acupoints were punctured once every 2 days for 7 times.The mRNA expression of signal transducer and activator of transcription 6(STAT6),the protein and positive expression of transforming growth factor-β1(TGF-β1),and the levels ofα-SMA,FN and peripheral blood T lymphocyte populations(CD3+,CD4+,CD8+)in the lung tissues of the four groups were compared.Results Compared with the normal control group,STAT6 mRNA expression,TGF-β1 protein and positive expression,the positive expression ofα-SMA,FN and T lymphocyte populations(CD3+,CD8+)in peripheral blood in model group were significantly increased[STAT6 mRNA(2-ΔΔCt):13.12±2.20 vs.1.02±0.20,TGF-β1 protein expression(A value):0.10±0.01 vs.0.06±0.01,TGF-β1 positive expression(A value):0.11±0.01 vs.0.08±0.01,α-SMA positive expression(A value):19.65±1.37 vs.7.74±0.81,FN positive expression(A value):18.40±0.79 vs.6.50±0.60,CD3+:0.70±0.03 vs.0.59±0.02,CD8+:0.39±0.02 vs.0.20±0.02,all P<0.05],and CD4+in model group was significantly decreased(0.32±0.02 vs.0.39±0.03,P<0.05);STAT6 mRNA expression and TGF-β1 protein expression,the positive expression ofα-SMA and FN and T lymphocyte populations(CD3+,CD8+)in peripheral blood in the drug group and acupuncture group were significantly lower than those in the model group,CD4+in the drug group and acupuncture group was significantly higher than that in the model group,and the changes in the acupuncture group was more significant than those in the drug group[STAT6 mRNA(2-ΔΔCt):3.01±0.55 vs.5.64±0.65,TGF-β1 protein expression(A value):0.06±0.01 vs.0.09±0.01,TGF-β1 positive expression(A value):0.08±0.01 vs.0.09±0.01,α-SMA positive expression(A value):12.33±0.50 vs.14.99±0.53,FN positive expression(A value):9.91±0.61 vs.13.19±0.66,CD3+:0.60±0.03 vs.0.65±0.04,CD4+:0.39±0.02 vs.0.36±0.02,CD8+:0.21±0.04 vs.0.28±0.03,all P<0.05].Conclusion For asthma model rats,acupuncture at Feishu,Dazhui and Fengmen acupoints can significantly stimulate the nerves and muscles of lung tissue,promote blood circulation in the lung,enhance lung function,improve the health status of lung tissue,reduce the content of the positive expression ofα-SMA and FN,and improve airway remodeling.