Effect of active components of Chinese herbal medicine Xiaoxuming decoction on oxidative stress and neuronal apoptosis in rats with chronic cerebral ischemia / 中华神经医学杂志

ABSTRACT

Objective To investigate the effect of active components of Xiaoxuming decoction (XXM) on oxidative stress and neuronal apoptosis in rats with chronic cerebral ischemia.Methods Bilateral common carotid arteries were permanently occluded in rats to establish the chronic cerebral ischemia models.The rats with chronic cerebral ischemia were randomly divided into five groupsvehicle group,Ginkgo biloba extract (EGB) group,and low-,medium-and high-dose active components of XXM groups.The sham-operated rats were treated with saline.The activities of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),catalase and the malondialdehyde (MDA) content were detected with spectrophotometry.TUNEL assay was used to measure the neuronal apoptosis.Western blotting was used to observe the expressions of neuronal apoptosis related proteins such as Caspase-3,Bcl-2 and Bax.Results The low-dose and medium-dose active components of XXM could significantly inhibit oxidative stress,decrease lipid peroxide,and attenuate the decreased activities of GSH-Px in 2VO rats (P＜0.05).The medium-dose active components of XXM markedly prevented neurons from apoptosis (P＜0.05).In addition,the low-dose and medium-dose active components of XXM increased Bcl-2/Bax ratio and decreased Caspase-3 mediated downstream cascade reaction (P＜0.05).Conclusion The active components of XXM can inhibit oxidative stress and decrease neuronal apoptosis in rots with chronic cerebral ischemia.