Preliminary research of acute brain injury after intraventricular hemorrhage / 中华神经医学杂志

ABSTRACT

Objective To investigate the pathological characteristics of acute brain injury in rat models of intraventricular hemorrhage (IVH).Methods Thirty-eight male Sprague-Dawley rats were randomly divided into control group and IVH group; stereotactic technique was employed to perform a 200-μL injection of either saline (control group) or autologous blood (IVH group) into the right lateral ventricle and these rats were euthanized at different time points.The weight loss of the rats was observed at 24 h after injection.The morphological changes of the cerebral ventricle were investigated using Micro-CT and brain samples.The brain water content by wet-dry weighting method was used to evaluate brain edema after IVH.Finally,blood brain barrier (BBB) injury was investigated by Evans blue (EB) staining (24 h after injection) and IgG immunofluorescent tests (48 h after injection).Results The weight loss in the IVH group was significantly more obvious than that in the control group 24 h after the injection (P＜0.05).Ventricular dilatation and cast form of ventricle were noted at the IVH group.The brain water content of the IVH group significantly increased in bilateral cerebral hemispheres as compared with that of the control group (P＜0.05).Evans blue exudation into the brain tissue and IgG expression in ventricle choroid plexus in the IVH group obviously increased as compared with those in the control group.Conclusion Besides hydrocephalus,acute brain injury after IVH might also accompany with acute ecephaledema and BBB damage,which are probably important pathological characteristics in acute brain injury after IVH.