Expression of Nav1.1 and its significance in brain tissues of FMR1 knockout mice / 中华神经医学杂志
Chinese Journal of Neuromedicine
; (12): 894-898, 2015.
Article
de Zh
| WPRIM
| ID: wpr-1034248
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To explore the possible role of Nav 1.1 in the pathogenesis of increased susceptibility to epileptic seizures in FMR1 knockout (FMR1 KO) mice.Methods FVB strain FMR1 KO mice and wild type (WT) controls at ages of 2 and 4 weeks old were chosen;immunohistochemistry was used to determine the expression of Nav 1.1 in different brain regions (striate cortex,temporal cortex,piriform cortex,hippocampus CA1,CA3 and dentate gyrus),and Western blotting was used to determine the Nav1.1 level in the cerebral acustici cortex and hippocampus.Results The mean optical density of Nav1.1 was significantly increased in the striate cortex,temporal acustici cortex,piriform cortex,regions of CA1 and dentate gyrus in FMR1 KO mice at ages of 2 and 4 weeks (2 weeks:0.058±0.006,0.054± 0.006,0.130±0.015,0.090±0.009 and 0.142±0.010;4 weeks:0.066±0.007,0.060±0.007,0.159±0.018,0.102±0.015 and 0.192±0.025) as compared with the age-matched WT mice (2 weeks:0.049±0.007,0.046±0.007,0.118±0.012,0.080±0.009 and 0.133±0.010;4 weeks:0.051±0.007,0.048±0.005,0.127± 0.012,0.089±0.012 and 0.175±0.024,P<0.05).The levels of Nav1.1 in the cerebra1 cortex and hippocampus in FMR1 KO mice at ages of 2 and 4 weeks (2 weeks:0.635±0.082 and 0.954±0.111;4 weeks:0.819 ±0.064 and 1.145 ±0.159) were also significantly increased as compared with the age-matched WT mice (2 weeks:0.382±0.025 and 0.555±0.056;4 weeks:0.550±0.040 and 0.847±0.127,P<0.05).Conclusion Increased expression of Nav1.1 might play an important role in the increased susceptibility to epileptic seizures in FMR1 KO mice.
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WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Neuromedicine
Année:
2015
Type:
Article