Transportation in extracellular space in the caudate nucleus of rat models of Parkinson's disease: a MR imaging study / 中华神经医学杂志

ABSTRACT

Objective To investigate the transportation alterations in extracellular space (ECS) in the caudate nucleus of rat models of Parkinson's disease (PD).Methods Twenty mature Sprague-Dawley rats were randomly divided into PD model group and control group (n=10).Rats in the model group received daily subcutaneous injection of 1.5 mg/mL rotenone solution at dosage of one mg/kg once daily for a consecutive 7 d,and PD was verified using immunohistochemistry and inclined plane tests;rats in the control group did not receive any treatment.Tracer-based magnetic resonance imaging (MRI) was used to quantitatively investigate the alterations of transportation in ECS.Results The mean tilt angle of rats in the model group was (34.5±3.9)°,and that in the control group was (43.1±3.8)°,with significant difference (t=4.994,P=0.002).Immunohistochemistry indicated that small amount of TH positive cells were noted in the model group,while large numbers of TH positive cells were noted in the control group.The transportation in ECS ofcaudate nucleus in the model group was demonstrated slower as compared with that in the control group,and half-time of tracer was significantly increased as compared with that in the control group ([156.6±10.2] min vs.[114.6±8.7] min,t=9.907,P=0.000).Conclusion Tracer-based MRI can be used to investigate the transportation in ECS in the deep brain regions of PD.