Regulatory and mechanism of miRNA-134 in animal models of hypoxic ischemic encephalopathy / 中华神经医学杂志

ABSTRACT

Objective To explore the neuro-functional regulation and mechanism ofmiRNA in neonatal hypoxic ischemic encephalopathy (HIE).Methods Sixty 7-d-old SD rats were randomly divided into a sham-operated group and a hypoxic ischemic group (n=30);HIE models were induced by modified Rice method.Real-time fluorescent quantitative PCR was employed to detect the miR-134 expression and immumohistochemical staining was used to detect the Limk1 expression in the brain tissues of two groups 0,1,and 7 d after hypoxia ischemia (10 rats in each time point).Results As compared with sham-operated group,hypoxic ischemic group had significantly lower miR-134 level (P＜0.05);and 0,1,and 7 d after hypoxia ischemia,the miR-134 level decreased in sequence,with significant differences between each two time points (P＜0.05).As compared with sham-operated group,hypoxic ischemic group had significantly higher Limk1 level (P＜0.05);and 0,1,and 7 d after hypoxia ischemia,the Limk1 level increased in sequence,with significant differences between each two time points (P＜0.05).Conclusion MiRNA-134 may play an important role in neuronal plasticity and development of nervous system in brain tissues of HIE by regulating Limk1 expression.