Effects of sFRP3 overexpression on the activation and proliferation of murine cardiac fibroblasts / 安徽医科大学学报

ABSTRACT

Objective @#To explore the role of secreted frizzled ⁃related protein 3 ( sFRP3) , a regulator of the Wnt signaling pathway , in the activation and proliferation of murine cardiac fibroblasts ( CFs ) . @*Methods @#Neonatalmice aged 1 -3 days were obtained for surgical procedures to collect heart tissues. After digestion, CFs were isola.ted and cultured. Transforming growth factor-beta 1 (TGF-β1 ) stimulation was used to induce activation and prolif.eration in CF's after they adhered to the culture dish. Once the model was confirmed, experimental and controlgroups were transfected with sFRP3 overexpression plasmids and empty plasmids for 24 -48 hours. Expression lev.els of sFRP3, Periostin (POSTN), Type I collagen ( Collagen I ), and proliferating cell nuclear antigen ( PCNA) were assessed at the molecular level using Westerm blot and qR'T-PCR. Changes in cell proliferation capacitywere examined using M'TT, CCK-8, and EdU staining methods.@*Results@#In the TGF-β1-induced activation andproliferation model of CFs, compared to the control group, the model group exhibited decreased expression of sFRP3 protein and mRNA , while the expression of activation and proliferation-related proteins PCNA, POSTN, andCollagen I was upregulated. Furthermore, in CFs overexpressing sFRP3 through plasmid transfection, the proteinand mRNA expression of PCNA, POSTN, and Collagen I decreased compared to the empty vector group. MTTCCK-8, and EdU experiments indicated a significant decrease in the proliferative activity of CFs in the sFRP3 over0verexpression of sFRP3 markedly inhibits theexpression group compared to the empty vector group. @*Conclusion@#Overexpression of sFRP3 markedly inhibits theactivation and proliferation of CFs, suggesting that sFRP3 may be a key gene involved in the regulation of CF activation and proliferation.